PRODUCT IDENTIFICATION

224-087-1

c12c3c(ccc1NC(C)(C)Nc2ccc3)\N=N\c1c2c(c(\N=N\c3ccccc 3)cc1)cccc2

Sudan stain

EXTRA NOTES

A nonfluorescent fat-soluble diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections.

PHYSICAL AND CHEMICAL PROPERTIES

Dark brown to black powder

AUTOIGNITION

REFRACTIVE INDEX

EXTERNAL LINKS & GENERAL DESCRIPTIONS

Wikipedia Linking - Sudan Black B

Google Scholar Search - Sudan Black B

Drug Information Portal (U.S. National Library of Medicine) - Sudan Black B

PubChem Compound Summary - Sudan Black B

KEGG (Kyoto Encyclopedia of Genes and Genomes) - Sudan Black B

http://www.ebi.ac.uk/ - Sudan Black B

http://www.ncbi.nlm.nih.gov/ - Sudan Black B

http://toxnet.nlm.nih.gov/
Hazardous Substances Data Bank - Sudan Black B

http://www.sigmaaldrich.com/
Sudan Black B was found to eliminate lipofuscin-like autofluorescence in mammalian neural tissue without adversely affecting other fluorescent labels. It was also found to reduce autofluroescence in archival formaldehyde-fixed paraffin-embedded myocardium tissue. Sudan Black B was used to detect native and oxidized low density lipoproteins (LDLs) after separation by capillary isotachophoresis (CITP). It has been used for staining fat in animal tissues and in bacteria (Burdon's method), as well as the lipid portion of lipoprotein in polyacrylamide gel electrophoresis

Local:
Sudan is a group of solvent dye, frequently called lysochrome (Lyso- means dissolving and -chrome means colour). In structural classification, sudan belongs to diazo dye. Azo colorant molecule can be described as aryl-N=N-R, where R is aryl, heteroaryl or -CH=C(OH)-. Solvent dyes are insoluble in water but soluble in nonpolar organic solvents and fats. It is usually used as a solution in an organic solvent. Azo structure solvent dyes are often responsible yellow-red color whereas anthraquinone structure solvent dyes tend to be green-blue. Some other structures for solvent dyes include heterocyclic rings, metal complex, pyrazolone, xanthene, and ketoamine. Solvent dyes do not ionise. Solvent dye, undergoing molecular rearrangement, is dissoluted in the substrate. It colours and binds to the lipids resulting in showing colored regions. In industrial field, it is used in coloring oils, waxes, greases, fats, hydrocarbons derivatives, polishes, oily insecticides, and acrylic emulsions. Sudans are used for demonstrating fats, triglycerides and lipoproteins present in tissues as biological stains.

• Sudan I [Solvent Orange R, CAS #: 842-07-9]; a yellow lysochrome; C.I. solvent yellow 14; carcinogen suspect. Chemically 1-phenylazo-2-naphthol.
• Sudan II [Solvent Orange 7, CAS #: 3118-97-6]; an orange lysochrome; C.I. Solvent Orange 7; carcinogen suspect. Chemical designation 1-(2,4-Dimethylphenylazo)-2-naphthol
• Sudan III [Sudan Red, CAS #: 85-86-9] A red lysochrome; C.I. Solvent Red 23; used as a stain for the demonstration of triglycerides and fats. Chemical designation 1-[4-(Phenylazo)phenylazo]-2-naphthol.
• Sudan IV [Scarlet Red, CAS #: 85-83-6] A scarlet red lysochrome; Solvent Red 24; used as a stain for the demonstration of triglycerides and fats. deeper stain than Sudan III,Chemical designation 1-[[2-methyl-4-[(2-methylphenyl)azo]phenyl]azo]-2-Naphthalenol
• Sudan Black B [CAS #: 4197-25-5],  a black lysochrome; Solvent Black 3; used as a stain for the demonstration of fat vacuoles in bacterial cells. Cleared with xylol and counterstained with safranin. Chemical designation 2,3-Dihydro-2,2-dimethyl-6-[[4-(phenylazo)-1-naphthyl]azo]-1H-Perimidine.

APPEARANCE

Dark brown to black powder

SUDAN PRODUCTS